Manage a cell type registry

Background

Cell types classify cells based on public and private knowledge gained from studying gene expression patterns, morphology, functional & other properties.

Long established cell types have known markers and properties but cell subtypes and states are continuously being discovered, better understood and knowledge gets refined.

In this notebook, we use CellTypist, a computational tool for cell type classification in scRNA-seq data. It assigns cell types based on gene expression profiles.

First, we create a cell type registry for cell types supported by CellTypist.

Then, we’ll use CellTypist to classify cell types of a previously unannotated dataset and ingest the dataset with LaminDB.

Finally, we will demonstrate how to fetch datasets with cell type queries using LaminDB.

Setup

To run this notebook, you need to load a LaminDB instance that has the bionty schema mounted.

Here, we’ll create a test instance (skip if you’d like to run it using your instance):

Show code cell content Hide code cell content

!lamin init --storage ./celltypist --schema bionty

💡 creating schemas: core==0.45.2 bionty==0.29.3 
🌱 saved: User(id='DzTjkKse', handle='testuser1', email='testuser1@lamin.ai', name='Test User1', updated_at=2023-08-12 05:42:07)
🌱 saved: Storage(id='ATyyz7R2', root='/home/runner/work/lamin-usecases/lamin-usecases/docs/celltypist', type='local', updated_at=2023-08-12 05:42:07, created_by_id='DzTjkKse')

✅ loaded instance: testuser1/celltypist
💡 did not register local instance on hub (if you want, call `lamin register`)

Show code cell content Hide code cell content

# filter warnings from celltypist
import warnings

warnings.filterwarnings("ignore", message=".*The 'nopython' keyword.*")

import lamindb as ln
import lnschema_bionty as lb
import celltypist
import pandas as pd

lb.settings.species = "human"  # globally set species

✅ loaded instance: testuser1/celltypist (lamindb 0.50.3)

🌱 set species: Species(id='uHJU', name='human', taxon_id=9606, scientific_name='homo_sapiens', updated_at=2023-08-12 05:42:11, bionty_source_id='n5t1', created_by_id='DzTjkKse')

ln.track()

💡 notebook imports: celltypist==1.6.0 lamindb==0.50.3 lnschema_bionty==0.29.3 pandas==2.0.3

🌱 saved: Transform(id='s5mkN5NQ1ttIz8', name='Manage a cell type registry', short_name='celltypist', stem_id='s5mkN5NQ1ttI', version='0', type=notebook, updated_at=2023-08-12 05:42:11, created_by_id='DzTjkKse')

🌱 saved: Run(id='UKB2bEgIHN2115EtV0it', run_at=2023-08-12 05:42:11, transform_id='s5mkN5NQ1ttIz8', created_by_id='DzTjkKse')

For a start, let’s take a look at the public Cell Ontology.

celltype_bt = lb.CellType.bionty()  # equals to bionty.CellType()
celltype_bt

CellType
Species: all
Source: cl, 2023-04-20
#terms: 2862

📖 CellType.df(): ontology reference table
🔎 CellType.lookup(): autocompletion of terms
🎯 CellType.search(): free text search of terms
✅ CellType.validate(): strictly validate values
🧐 CellType.inspect(): full inspection of values
👽 CellType.map_synonyms(): map synonyms to standardized names
🪜 CellType.diff(): difference between two versions
🔗 CellType.ontology: Pronto.Ontology object

Create an in-house registry of CellTypist terms based on the public Cell Ontology

Fetch CellTypist’s immune cell encyclopedia

As a first step we will read in CellTypist’s immune cell encyclopedia

description = "CellTypist Pan Immune Atlas v2: basic cell type information"
celltypist_source_v2_url = "https://github.com/Teichlab/celltypist_wiki/raw/main/atlases/Pan_Immune_CellTypist/v2/tables/Basic_celltype_information.xlsx"

# our source data
celltypist_file = ln.File.filter(description=description).one_or_none()

if celltypist_file is None:
    celltypist_df = pd.read_excel(celltypist_source_v2_url)
    celltypist_file = ln.File(celltypist_df).save()
else:
    celltypist_df = celltypist_file.load().head()

💡 file will be copied to default storage upon `save()` with key 'd6klF92VDHi6PTGtjNSi.parquet'

💡 file is a dataframe, consider using File.from_df() to link column names as features

🌱 storing file 'd6klF92VDHi6PTGtjNSi' with key '.lamindb/d6klF92VDHi6PTGtjNSi.parquet'

It provides an ontology_id of the public Cell Ontology for the majority of records.

celltypist_df.head()

	High-hierarchy cell types	Low-hierarchy cell types	Description	Cell Ontology ID	Curated markers
0	B cells	B cells	B lymphocytes with diverse cell surface immuno...	CL:0000236	CD79A, MS4A1, CD19
1	B cells	Follicular B cells	resting mature B lymphocytes found in the prim...	CL:0000843	CXCR5, TNFRSF13B, CD22
2	B cells	Proliferative germinal center B cells	proliferating germinal center B cells	CL:0000844	MKI67, SUGCT, AICDA
3	B cells	Germinal center B cells	proliferating mature B cells that undergo soma...	CL:0000844	POU2AF1, CD40, SUGCT
4	B cells	Memory B cells	long-lived mature B lymphocytes which are form...	CL:0000787	CR2, CD27, MS4A1

The “Cell Ontology ID” is associated with multiple “Low-hierarchy cell types”:

celltypist_df.set_index(["Cell Ontology ID", "Low-hierarchy cell types"]).head(10)

		High-hierarchy cell types	Description	Curated markers
Cell Ontology ID	Low-hierarchy cell types
CL:0000236	B cells	B cells	B lymphocytes with diverse cell surface immuno...	CD79A, MS4A1, CD19
CL:0000843	Follicular B cells	B cells	resting mature B lymphocytes found in the prim...	CXCR5, TNFRSF13B, CD22
CL:0000844	Proliferative germinal center B cells	B cells	proliferating germinal center B cells	MKI67, SUGCT, AICDA
	Germinal center B cells	B cells	proliferating mature B cells that undergo soma...	POU2AF1, CD40, SUGCT
CL:0000787	Memory B cells	B cells	long-lived mature B lymphocytes which are form...	CR2, CD27, MS4A1
	Age-associated B cells	B cells	CD11c+ T-bet+ memory B cells associated with a...	FCRL2, ITGAX, TBX21
CL:0000788	Naive B cells	B cells	mature B lymphocytes which express cell-surfac...	IGHM, IGHD, TCL1A
CL:0000818	Transitional B cells	B cells	immature B cell precursors in the bone marrow ...	CD24, MYO1C, MS4A1
CL:0000817	Large pre-B cells	B-cell lineage	proliferative B lymphocyte precursors derived ...	MME, CD24, MKI67
	Small pre-B cells	B-cell lineage	non-proliferative B lymphocyte precursors deri...	MME, CD24, IGLL5

Inspect mapability with the public Cell Ontology

For any cell type record that can be mapped against the public Cell Ontology, we’d like to ensure that it’s actually mapped.

This will avoid that we’ll refer to the same cell type with different identifiers.

Let’s see how well the Cell Typist reference data can be mapped.

All Celltypist labeled ontology IDs are mappable to the public Cell Ontology:

celltype_bt.inspect(celltypist_df["Cell Ontology ID"], celltype_bt.ontology_id);

🔶 received 68 unique terms, 30 empty/duplicated terms are ignored

✅ 68 terms (100.00%) are validated

However, when inspecting the names, most of them don’t match:

celltype_bt.inspect(celltypist_df["Low-hierarchy cell types"], celltype_bt.name);

✅ 1 terms (1.00%) are validated

🔶 97 terms (99.00%) are not validated
   🟠 detected synonyms
   to increase validated terms, standardize them via .map_synonyms()

A search tells us that terms that are named in plural in Cell Typist occur with a name in singular in the Cell Ontology:

celltypist_df["Low-hierarchy cell types"][0]

'B cells'

celltype_bt.search(celltypist_df["Low-hierarchy cell types"][0]).head()

	ontology_id	definition	synonyms	parents	__agg__	__ratio__
name
B cell	CL:0000236	A Lymphocyte Of B Lineage That Is Capable Of B...	B-cell|B lymphocyte|B-lymphocyte	[CL:0000945]	b cell	92.307692
B-1 B cell	CL:0000819	A B Cell Of Distinct Lineage And Surface Marke...	B1 B cell|B-1 B lymphocyte|B1 cell|B-1 B-cell|...	[CL:0000785]	b-1 b cell	85.714286
B-2 B cell	CL:0000822	A Conventional B Cell Subject To Antigenic Sti...	B2 B cell|B2 B-lymphocyte|B2 B lymphocyte|B-2 ...	[CL:0000785]	b-2 b cell	85.714286
arachnoid barrier cell	CL:4023097	A Mesothelial Fibroblast Of The Arachnoid Barr...	ABC|AB cell	[CL:4023054]	arachnoid barrier cell	85.714286
Be cell	CL:0000968	A Mature B Cell That Produces Cytokines That C...	B effector cell|effector B-cell|effector B cel...	[CL:0000785]	be cell	85.714286

Let’s try to strip "s" and inspect if more names are mappable. Yes, there are!

celltype_bt.inspect(
    [i.rstrip("s") for i in celltypist_df["Low-hierarchy cell types"]],
    celltype_bt.name,
);

✅ 5 terms (5.10%) are validated

🔶 93 terms (94.90%) are not validated
   🟠 detected inconsistent casing
   🟠 detected synonyms
   to increase validated terms, standardize them via .map_synonyms()

Every “low-hierarchy cell type” has an ontology id and most “high-hierarchy cell types” also appear as “low-hierarchy cell types” in the Cell Typist table. Four, however, don’t, and therefore don’t have an ontology ID.

high_terms = celltypist_df["High-hierarchy cell types"].unique()
low_terms = celltypist_df["Low-hierarchy cell types"].unique()

high_terms_umapped = set(high_terms).difference(low_terms)
high_terms_umapped

{'B-cell lineage', 'Cycling cells', 'Erythroid', 'T cells'}

Register CellTypist records in LaminDB

Let’s first add the “High-hierarchy cell types” as a column "parent".

This enables LaminDB to populate the parents and children fields, which will enable you to query for hierarchical relationships.

celltypist_df["parent"] = celltypist_df.pop("High-hierarchy cell types")

# if high and low terms are the same, no parents
celltypist_df.loc[
    (celltypist_df["parent"] == celltypist_df["Low-hierarchy cell types"]), "parent"
] = None

# rename columns, drop markers
celltypist_df.drop(columns=["Curated markers"], inplace=True)
celltypist_df.rename(
    columns={"Low-hierarchy cell types": "name", "Cell Ontology ID": "ontology_id"},
    inplace=True,
)
celltypist_df.columns = celltypist_df.columns.str.lower()

celltypist_df.head(2)

	name	description	ontology_id	parent
0	B cells	B lymphocytes with diverse cell surface immuno...	CL:0000236	None
1	Follicular B cells	resting mature B lymphocytes found in the prim...	CL:0000843	B cells

Now, let’s create records from the public ontology:

public_records = lb.CellType.from_values(
    celltypist_df.ontology_id, lb.CellType.ontology_id
)

✅ validated 68 CellType records from Bionty on ontology_id: CL:0000236, CL:0000843, CL:0000844, CL:0000787, CL:0000788, CL:0000818, CL:0000817, CL:0002046, CL:0000826, CL:0001056, CL:0000798, CL:0000576, CL:0000623, CL:0000084, CL:0000990, CL:0000840, CL:0001029, CL:0002489, CL:0000809, CL:0000553, ...

Let’s now amend public ontology records so that they maintain additional annotations that Cell Typist might have.

records_names = {}
public_records_dict = {r.ontology_id: r for r in public_records}

for _, row in celltypist_df.iterrows():
    name = row["name"]
    ontology_id = row["ontology_id"]
    public_record = public_records_dict[ontology_id]

    # if both name and ontology_id match public record, use public record
    if name.lower() == public_record.name.lower():
        records_names[name] = public_record
        continue
    else:  # when ontology_id matches the public record and name doesn't match
        # if singular form of the Celltypist name matches public name
        if name.lower().rstrip("s") == public_record.name.lower():
            # add the Celltypist name to the synonyms of the public ontology record
            public_record.add_synonym(name)
            records_names[name] = public_record
            continue
        if public_record.synonyms is not None:
            synonyms = [s.lower() for s in public_record.synonyms.split("|")]
            # if any of the public matches celltypist name
            if any(
                [
                    i.lower() in {name.lower(), name.lower().rstrip("s")}
                    for i in synonyms
                ]
            ):
                # add the Celltypist name to the synonyms of the public ontology record
                public_record.add_synonym(name)
                records_names[name] = public_record
                continue

        # create a record only based on Celltypist metadata
        records_names[name] = lb.CellType(
            name=name, ontology_id=ontology_id, description=row.description
        )

You can see certain records are created by adding the Celltypist name to the synonyms of the public record:

records_names["GMP"]

CellType(id='f5eAsw0p', name='granulocyte monocyte progenitor cell', ontology_id='CL:0000557', synonyms='CFU-GM|granulocyte/monocyte precursor|colony forming unit granulocyte macrophage|GMP|granulocyte-macrophage progenitor|granulocyte/monocyte progenitor', description='A Hematopoietic Progenitor Cell That Is Committed To The Granulocyte And Monocyte Lineages. These Cells Are Cd123-Positive, And Do Not Express Gata1 Or Gata2 But Do Express C/Ebpa, And Pu.1.', bionty_source_id='Le97', created_by_id='DzTjkKse')

Other records are created based on Celltypist metadata:

records_names["Age-associated B cells"]

CellType(id='00ieV0IG', name='Age-associated B cells', ontology_id='CL:0000787', description='CD11c+ T-bet+ memory B cells associated with autoimmunity and aging', created_by_id='DzTjkKse')

Let’s save them to our database:

records = set(records_names.values())

ln.save(records)

Show code cell output Hide code cell output

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='nd6Qaf38', name='Hofbauer cell', ontology_id='CL:3000001', synonyms='Hofbauer cells', description='Oval Eosinophilic Histiocytes With Granules And Vacuoles Found In Placenta, Which Are Of Mesenchymal Origin, In Mesoderm Of The Chorionic Villus, Particularly Numerous In Early Pregnancy.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 4 CellType records on ontology_id: CL:0000235, CL:0000235, CL:0000235, CL:0000235

💡 also saving parents of CellType(id='loo3Xanl', name='common myeloid progenitor', ontology_id='CL:0000049', synonyms='common myeloid precursor|CMP', description='A Progenitor Cell Committed To Myeloid Lineage, Including The Megakaryocyte And Erythroid Lineages.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0002032

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='JYQl3RX8', name='hematopoietic oligopotent progenitor cell', ontology_id='CL:0002032', description='A Hematopoietic Oligopotent Progenitor Cell That Has The Ability To Differentiate Into Limited Cell Types But Lacks Lineage Cell Markers And Self Renewal Capabilities.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 2 CellType records from Bionty on ontology_id: CL:0008001, CL:0000988

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='Q0aQr5JB', name='hematopoietic cell', ontology_id='CL:0000988', synonyms='haematopoietic cell|hemopoietic cell|haemopoietic cell', description='A Cell Of A Hematopoietic Lineage.', updated_at=2023-08-12 05:42:14, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 2 CellType records from Bionty on ontology_id: CL:0002371, CL:0000548

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='H0taCt24', name='animal cell', ontology_id='CL:0000548', synonyms='metazoan cell', description='A Native Cell That Is Part Of Some Metazoa.', updated_at=2023-08-12 05:42:14, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0000255

💡 also saving parents of CellType(id='QMAH6IlS', name='somatic cell', ontology_id='CL:0002371', description='A Cell Of An Organism That Does Not Pass On Its Genetic Material To The Organism'S Offspring (I.E. A Non-Germ Line Cell).', updated_at=2023-08-12 05:42:14, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0000548

✅ validated 1 CellType record from Bionty on ontology_id: CL:0000003

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='VT73gpK2', name='native cell', ontology_id='CL:0000003', description='A Cell That Is Found In A Natural Setting, Which Includes Multicellular Organism Cells 'In Vivo' (I.E. Part Of An Organism), And Unicellular Organisms 'In Environment' (I.E. Part Of A Natural Environment).', updated_at=2023-08-12 05:42:14, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0000000

💡 also saving parents of CellType(id='0d3ym06W', name='hematopoietic precursor cell', ontology_id='CL:0008001', description='Any Hematopoietic Cell That Is A Precursor Of Some Other Hematopoietic Cell Type.', updated_at=2023-08-12 05:42:14, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0000988

💡 also saving parents of CellType(id='Z7uMAWUF', name='regulatory T cell', ontology_id='CL:0000815', synonyms='Treg|regulatory T-lymphocyte|regulatory T-cell|regulatory T lymphocyte|Regulatory T cells', description='A T Cell Which Regulates Overall Immune Responses As Well As The Responses Of Other T Cell Subsets Through Direct Cell-Cell Contact And Cytokine Release.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0002419

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='2C5PhwrW', name='mature T cell', ontology_id='CL:0002419', synonyms='mature T-cell|CD3e-positive T cell', description='A T Cell That Expresses A T Cell Receptor Complex And Has Completed T Cell Selection.', updated_at=2023-08-12 05:42:15, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 2 CellType records on ontology_id: CL:0000084, CL:0000084

💡 also saving parents of CellType(id='4fOuOYtl', name='endothelial cell', ontology_id='CL:0000115', synonyms='endotheliocyte|Endothelial cells', description='An Endothelial Cell Comprises The Outermost Layer Or Lining Of Anatomical Structures And Can Be Squamous Or Cuboidal. In Mammals, Endothelial Cell Has Vimentin Filaments And Is Derived From The Mesoderm.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 2 CellType records from Bionty on ontology_id: CL:0000213, CL:0002078

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='AHV57RuN', name='lining cell', ontology_id='CL:0000213', synonyms='boundary cell', description='A Cell Within An Epithelial Cell Sheet Whose Main Function Is To Act As An Internal Or External Covering For A Tissue Or An Organism.', updated_at=2023-08-12 05:42:15, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0000215

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='gON03kRx', name='barrier cell', ontology_id='CL:0000215', description='A Cell Whose Primary Function Is To Prevent The Transport Of Stuff Across Compartments.', updated_at=2023-08-12 05:42:16, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0000003

💡 also saving parents of CellType(id='nGEtVlKq', name='meso-epithelial cell', ontology_id='CL:0002078', synonyms='epithelial mesenchymal cell', description='Epithelial Cell Derived From Mesoderm Or Mesenchyme.', updated_at=2023-08-12 05:42:15, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0002371

💡 also saving parents of CellType(id='FMTngXKK', name='follicular B cell', ontology_id='CL:0000843', synonyms='follicular B-cell|Fo B-cell|follicular B lymphocyte|follicular B-lymphocyte|Follicular B cells|Fo B cell', description='A Resting Mature B Cell That Has The Phenotype Igm-Positive, Igd-Positive, Cd23-Positive And Cd21-Positive, And Found In The B Cell Follicles Of The White Pulp Of The Spleen Or The Corticol Areas Of The Peripheral Lymph Nodes. This Cell Type Is Also Described As Being Cd19-Positive, B220-Positive, Aa4-Negative, Cd43-Negative, And Cd5-Negative.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0000785

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='0I51jgPp', name='mature B cell', ontology_id='CL:0000785', synonyms='mature B lymphocyte|mature B-cell|mature B-lymphocyte', description='A B Cell That Is Mature, Having Left The Bone Marrow. Initially, These Cells Are Igm-Positive And Igd-Positive, And They Can Be Activated By Antigen.', updated_at=2023-08-12 05:42:16, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0001201

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='CIS4VJI0', name='B cell, CD19-positive', ontology_id='CL:0001201', synonyms='CD19+ B cell|B lymphocyte, CD19-positive|B-lymphocyte, CD19-positive|CD19-positive B cell|B-cell, CD19-positive', description='A B Cell That Is Cd19-Positive.', updated_at=2023-08-12 05:42:16, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 2 CellType records on ontology_id: CL:0000236, CL:0000236

💡 also saving parents of CellType(id='cx8VcggA', name='B cell', ontology_id='CL:0000236', synonyms='B-cell|B cells|B lymphocyte|B-lymphocyte', description='A Lymphocyte Of B Lineage That Is Capable Of B Cell Mediated Immunity.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0000945

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='Z0yFV7vU', name='lymphocyte of B lineage', ontology_id='CL:0000945', description='A Lymphocyte Of B Lineage With The Commitment To Express An Immunoglobulin Complex.', updated_at=2023-08-12 05:42:17, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0000542

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='g8slxY8X', name='lymphocyte', ontology_id='CL:0000542', description='A Lymphocyte Is A Leukocyte Commonly Found In The Blood And Lymph That Has The Characteristics Of A Large Nucleus, A Neutral Staining Cytoplasm, And Prominent Heterochromatin.', updated_at=2023-08-12 05:42:17, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0000738

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='MkrH0gsX', name='leukocyte', ontology_id='CL:0000738', synonyms='white blood cell|leucocyte', description='An Achromatic Cell Of The Myeloid Or Lymphoid Lineages Capable Of Ameboid Movement, Found In Blood Or Other Tissue.', updated_at=2023-08-12 05:42:17, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0000988

💡 also saving parents of CellType(id='RSjQq98q', name='pro-B cell', ontology_id='CL:0000826', synonyms='Pro-B cells|progenitor B-lymphocyte|progenitor B cell|progenitor B lymphocyte|pro-B-lymphocyte|pro-B lymphocyte|progenitor B-cell|pro-B-cell', description='A Progenitor Cell Of The B Cell Lineage, With Some Lineage Specific Activity Such As Early Stages Of Recombination Of B Cell Receptor Genes, But Not Yet Fully Committed To The B Cell Lineage Until The Expression Of Pax5 Occurs.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0000838

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='vQ9N8BKH', name='lymphoid lineage restricted progenitor cell', ontology_id='CL:0000838', description='A Progenitor Cell Restricted To The Lymphoid Lineage.', updated_at=2023-08-12 05:42:18, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0002031

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='xl6RtpG9', name='hematopoietic lineage restricted progenitor cell', ontology_id='CL:0002031', description='A Hematopoietic Progenitor Cell That Is Capable Of Developing Into Only One Lineage Of Hematopoietic Cells.', updated_at=2023-08-12 05:42:18, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 2 CellType records on ontology_id: CL:0000988, CL:0008001

💡 also saving parents of CellType(id='ePsFBu6n', name='transitional stage B cell', ontology_id='CL:0000818', synonyms='transitional B cell|transitional stage B lymphocyte|Transitional B cells|transitional stage B-cell|transitional stage B-lymphocyte', description='An Immature B Cell Of An Intermediate Stage Between The Pre-B Cell Stage And The Mature Naive Stage With The Phenotype Surface Igm-Positive And Cd19-Positive, And Are Subject To The Process Of B Cell Selection. A Transitional B Cell Migrates From The Bone Marrow Into The Peripheral Circulation, And Then To The Spleen.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0001201

💡 also saving parents of CellType(id='l0R9X3Bs', name='promyelocyte', ontology_id='CL:0000836', synonyms='Promyelocytes', description='A Precursor In The Granulocytic Series, Being A Cell Intermediate In Development Between A Myeloblast And Myelocyte, That Has Distinct Nucleoli, A Nuclear-To-Cytoplasmic Ratio Of 5:1 To 3:1, And Containing A Few Primary Cytoplasmic Granules. Markers For This Cell Are Fucosyltransferase Fut4-Positive, Cd33-Positive, Integrin Alpha-M-Negative, Low Affinity Immunoglobulin Gamma Fc Region Receptor Iii-Negative, And Cd24-Negative.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0002191

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='odstSt5D', name='granulocytopoietic cell', ontology_id='CL:0002191', description='A Cell Involved In The Formation Of A Granulocyte.', updated_at=2023-08-12 05:42:18, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0000839

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='Q0v4wWyZ', name='myeloid lineage restricted progenitor cell', ontology_id='CL:0000839', description='A Progenitor Cell Restricted To The Myeloid Lineage.', updated_at=2023-08-12 05:42:19, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0002031

💡 also saving parents of CellType(id='YN0gzDt3', name='Kupffer cell', ontology_id='CL:0000091', synonyms='littoral cell of hepatic sinusoid|macrophagocytus stellatus|Kupffer cells|von Kupffer cell|hepatic macrophage|liver macrophage|stellate cell of von Kupffer', description='A Tissue-Resident Macrophage Of The Reticuloendothelial System Found On The Luminal Surface Of The Hepatic Sinusoids Involved In Erythrocyte Clearance. Markers Include F4/80+, Cd11B-Low, Cd68-Positive, Sialoadhesin-Positive, Cd163/Srcr-Positive. Irregular, With Long Processes Including Lamellipodia Extending Into The Sinusoid Lumen, Have Flattened Nucleus With Cytoplasm Containing Characteristic Invaginations Of The Plasma Membrane (Vermiform Bodies); Lie Within The Sinusoid Lumen Attached To The Endothelial Surface; Derived From The Bone Marrow, Form A Major Part Of The Body'S Mononuclear Phagocyte System.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0000864

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='pXnRaLwJ', name='tissue-resident macrophage', ontology_id='CL:0000864', synonyms='resting histiocyte|fixed macrophage', description='A Macrophage Constitutively Resident In A Particular Tissue Under Non-Inflammatory Conditions, And Capable Of Phagocytosing A Variety Of Extracellular Particulate Material, Including Immune Complexes, Microorganisms, And Dead Cells.', updated_at=2023-08-12 05:42:19, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 4 CellType records on ontology_id: CL:0000235, CL:0000235, CL:0000235, CL:0000235

💡 also saving parents of CellType(id='Iywg7lUq', name='early lymphoid progenitor', ontology_id='CL:0000936', synonyms='LMPP|lymphoid-primed multipotent progenitor|ELP', description='A Lymphoid Progenitor Cell That Is Found In Bone Marrow, Gives Rise To B Cells, T Cells, Natural Killer Cells And Dendritic Cells, And Has The Phenotype Lin-Negative, Kit-Positive, Sca-1-Positive, Flt3-Positive, Cd34-Positive, Cd150 Negative, And Glya-Negative.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0000837

💡 also saving parents of CellType(id='X458vtJX', name='naive B cell', ontology_id='CL:0000788', synonyms='naive B-lymphocyte|naive B-cell|naive B lymphocyte|Naive B cells', description='A Naive B Cell Is A Mature B Cell That Has The Phenotype Surface Igd-Positive, Surface Igm-Positive, Cd20-Positive, Cd27-Negative And That Has Not Yet Been Activated By Antigen In The Periphery.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0000785

💡 also saving parents of CellType(id='Q2BH279Q', name='classical monocyte', ontology_id='CL:0000860', synonyms='Classical monocytes|inflammatory monocyte', description='A Monocyte That Responds Rapidly To Microbial Stimuli By Secreting Cytokines And Antimicrobial Factors And Which Is Characterized By High Expression Of Ccr2 In Both Rodents And Humans, Negative For The Lineage Markers Cd3, Cd19, And Cd20, And Of Larger Size Than Non-Classical Monocytes.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 3 CellType records on ontology_id: CL:0000576, CL:0000576, CL:0000576

💡 also saving parents of CellType(id='xfxkvliE', name='granulocyte', ontology_id='CL:0000094', synonyms='polymorphonuclear leukocyte|granular leukocyte|Granulocytes|granular leucocyte', description='A Leukocyte With Abundant Granules In The Cytoplasm.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0000766

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='40onq0tm', name='myeloid leukocyte', ontology_id='CL:0000766', description='A Cell Of The Monocyte, Granulocyte, Or Mast Cell Lineage.', updated_at=2023-08-12 05:42:19, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0000738

💡 also saving parents of CellType(id='TENASE93', name='alveolar macrophage', ontology_id='CL:0000583', synonyms='Alveolar macrophages|dust cell', description='A Tissue-Resident Macrophage Found In The Alveoli Of The Lungs. Ingests Small Inhaled Particles Resulting In Degradation And Presentation Of The Antigen To Immunocompetent Cells. Markers Include F4/80-Positive, Cd11B-/Low, Cd11C-Positive, Cd68-Positive, Sialoadhesin-Positive, Dectin-1-Positive, Mr-Positive, Cx3Cr1-Negative.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0000864

✅ validated 1 CellType record from Bionty on ontology_id: CL:1001603

💡 also saving parents of CellType(id='P6E7yrc7', name='epithelial cell', ontology_id='CL:0000066', synonyms='epitheliocyte|Epithelial cells', description='A Cell That Is Usually Found In A Two-Dimensional Sheet With A Free Surface. The Cell Has A Cytoskeleton That Allows For Tight Cell To Cell Contact And For Cell Polarity Where Apical Part Is Directed Towards The Lumen And The Basal Part To The Basal Lamina.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0000548

💡 also saving parents of CellType(id='NJ07Q1hX', name='plasmablast', ontology_id='CL:0000980', synonyms='Plasmablasts|CD27-positive, CD38-positive, CD20-negative B cell', description='An Activated Mature (Naive Or Memory) B Cell That Is Secreting Immunoglobulin, Typified By Being Cd27-Positive, Cd38-Positive, Cd138-Negative.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0000785

💡 also saving parents of CellType(id='64kIG7So', name='gamma-delta T cell', ontology_id='CL:0000798', synonyms='gamma-delta T-lymphocyte|gamma-delta T lymphocyte|gamma-delta T-cell|gammadelta T cell|gamma-delta T cells', description='A T Cell That Expresses A Gamma-Delta T Cell Receptor Complex.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 2 CellType records on ontology_id: CL:0000084, CL:0000084

💡 also saving parents of CellType(id='XjG8T0GY', name='fibroblast', ontology_id='CL:0000057', synonyms='Fibroblasts', description='A Connective Tissue Cell Which Secretes An Extracellular Matrix Rich In Collagen And Other Macromolecules. Flattened And Irregular In Outline With Branching Processes; Appear Fusiform Or Spindle-Shaped.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0002320

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='4zAzIMBQ', name='connective tissue cell', ontology_id='CL:0002320', description='A Cell Of The Supporting Or Framework Tissue Of The Body, Arising Chiefly From The Embryonic Mesoderm And Including Adipose Tissue, Cartilage, And Bone.', updated_at=2023-08-12 05:42:20, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 2 CellType records on ontology_id: CL:0000548, CL:0002371

💡 also saving parents of CellType(id='ppLUhJWx', name='non-classical monocyte', ontology_id='CL:0000875', synonyms='Non-classical monocytes|resident monocyte|patrolling monocyte', description='A Type Of Monocyte Characterized By Low Expression Of Ccr2, Low Responsiveness To Monocyte Chemoattractant Ccl2/Mcp1, Low Phagocytic Activity, And Decrease Size Relative To Classical Monocytes, But Increased Co-Stimulatory Activity. May Also Play A Role In Tissue Repair.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 3 CellType records on ontology_id: CL:0000576, CL:0000576, CL:0000576

💡 also saving parents of CellType(id='8lTrmDbK', name='neutrophil', ontology_id='CL:0000775', synonyms='neutrophilic leukocyte|neutrophil leukocyte|Neutrophils|neutrophilic leucocyte|neutrophil leucocyte|neutrocyte', description='Any Of The Immature Or Mature Forms Of A Granular Leukocyte That In Its Mature Form Has A Nucleus With Three To Five Lobes Connected By Slender Threads Of Chromatin, And Cytoplasm Containing Fine Inconspicuous Granules And Stainable By Neutral Dyes.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0000094

💡 also saving parents of CellType(id='S4Urkinl', name='macrophage', ontology_id='CL:0000235', synonyms='Macrophages|histiocyte', description='A Mononuclear Phagocyte Present In Variety Of Tissues, Typically Differentiated From Monocytes, Capable Of Phagocytosing A Variety Of Extracellular Particulate Material, Including Immune Complexes, Microorganisms, And Dead Cells.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0000766

💡 also saving parents of CellType(id='f5eAsw0p', name='granulocyte monocyte progenitor cell', ontology_id='CL:0000557', synonyms='CFU-GM|granulocyte/monocyte precursor|colony forming unit granulocyte macrophage|GMP|granulocyte-macrophage progenitor|granulocyte/monocyte progenitor', description='A Hematopoietic Progenitor Cell That Is Committed To The Granulocyte And Monocyte Lineages. These Cells Are Cd123-Positive, And Do Not Express Gata1 Or Gata2 But Do Express C/Ebpa, And Pu.1.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0002032

💡 also saving parents of CellType(id='67zMsufW', name='memory B cell', ontology_id='CL:0000787', synonyms='memory B-lymphocyte|memory B lymphocyte|Memory B cells|memory B-cell', description='A Memory B Cell Is A Mature B Cell That Is Long-Lived, Readily Activated Upon Re-Encounter Of Its Antigenic Determinant, And Has Been Selected For Expression Of Higher Affinity Immunoglobulin. This Cell Type Has The Phenotype Cd19-Positive, Cd20-Positive, Mhc Class Ii-Positive, And Cd138-Negative.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0000785

💡 also saving parents of CellType(id='YzV7Qgmj', name='monocyte', ontology_id='CL:0000576', synonyms='Monocytes', description='Myeloid Mononuclear Recirculating Leukocyte That Can Act As A Precursor Of Tissue Macrophages, Osteoclasts And Some Populations Of Tissue Dendritic Cells.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0000766

💡 also saving parents of CellType(id='i20ionW5', name='mast cell', ontology_id='CL:0000097', synonyms='mastocyte|Mast cells|histaminocyte|labrocyte', description='A Cell That Is Found In Almost All Tissues Containing Numerous Basophilic Granules And Capable Of Releasing Large Amounts Of Histamine And Heparin Upon Activation. Progenitors Leave Bone Marrow And Mature In Connective And Mucosal Tissue. Mature Mast Cells Are Found In All Tissues, Except The Bloodstream. Their Phenotype Is Cd117-High, Cd123-Negative, Cd193-Positive, Cd200R3-Positive, And Fceri-High. Stem-Cell Factor (Kit-Ligand; Scf) Is The Main Controlling Signal Of Their Survival And Development.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0000766

✅ validated 1 CellType record from Bionty on ontology_id: CL:0002274

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='70wMh2r7', name='histamine secreting cell', ontology_id='CL:0002274', description='A Cell Type That Secretes Histamine.', updated_at=2023-08-12 05:42:20, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0000457

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='TpKvGjqi', name='biogenic amine secreting cell', ontology_id='CL:0000457', updated_at=2023-08-12 05:42:20, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0000151

💡 also saving parents of CellType(id='0JvRwfVm', name='plasma cell', ontology_id='CL:0000786', synonyms='plasma B-cell|plasmacyte|plasmocyte|Plasma cells|plasma B cell', description='A Terminally Differentiated, Post-Mitotic, Antibody Secreting Cell Of The B Cell Lineage With The Phenotype Cd138-Positive, Surface Immunonoglobulin-Negative, And Mhc Class Ii-Negative. Plasma Cells Are Oval Or Round With Extensive Rough Endoplasmic Reticulum, A Well-Developed Golgi Apparatus, And A Round Nucleus Having A Characteristic Cartwheel Heterochromatin Pattern And Are Devoted To Producing Large Amounts Of Immunoglobulin.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0000946

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='hFMWJcWc', name='antibody secreting cell', ontology_id='CL:0000946', description='A Lymphocyte Of B Lineage That Is Devoted To Secreting Large Amounts Of Immunoglobulin.', updated_at=2023-08-12 05:42:21, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0000945

💡 also saving parents of CellType(id='3rJgLble', name='conventional dendritic cell', ontology_id='CL:0000990', synonyms='dendritic reticular cell|DC1|type 1 DC|cDC', description='Conventional Dendritic Cell Is A Dendritic Cell That Is Cd11C-High.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record from Bionty on ontology_id: CL:0000451

🔶 now recursing through parents: this only happens once, but is much slower than bulk saving

💡 you can switch this off via: lb.settings.auto_save_parents = False

💡 also saving parents of CellType(id='9JGbXeUA', name='dendritic cell', ontology_id='CL:0000451', description='A Cell Of Hematopoietic Origin, Typically Resident In Particular Tissues, Specialized In The Uptake, Processing, And Transport Of Antigens To Lymph Nodes For The Purpose Of Stimulating An Immune Response Via T Cell Activation. These Cells Are Lineage Negative (Cd3-Negative, Cd19-Negative, Cd34-Negative, And Cd56-Negative).', updated_at=2023-08-12 05:42:21, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0000738

💡 also saving parents of CellType(id='g2Rk2xkb', name='myelocyte', ontology_id='CL:0002193', synonyms='Myelocytes', description='A Cell Type That Is The First Of The Maturation Stages Of The Granulocytic Leukocytes Normally Found In The Bone Marrow. Granules Are Seen In The Cytoplasm. The Nuclear Material Of The Myelocyte Is Denser Than That Of The Myeloblast But Lacks A Definable Membrane. The Cell Is Flat And Contains Increasing Numbers Of Granules As Maturation Progresses.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0002191

💡 also saving parents of CellType(id='uMLhrmbZ', name='germinal center B cell', ontology_id='CL:0000844', synonyms='germinal center B lymphocyte|germinal center B-lymphocyte|GC B-cell|GC B cell|Germinal center B cells|germinal center B-cell|GC B lymphocyte|GC B-lymphocyte', description='A Rapidly Cycling Mature B Cell That Has Distinct Phenotypic Characteristics And Is Involved In T-Dependent Immune Responses And Located Typically In The Germinal Centers Of Lymph Nodes. This Cell Type Expresses Ly77 After Activation.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0000785

💡 also saving parents of CellType(id='b5k0suF0', name='erythrocyte', ontology_id='CL:0000232', synonyms='RBC|Erythrocytes|red blood cell', description='A Red Blood Cell. In Mammals, Mature Erythrocytes Are Biconcave Disks Containing Hemoglobin Whose Function Is To Transport Oxygen.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 3 CellType records on ontology_id: CL:0000764, CL:0000764, CL:0000764

Add parent-child relationship of the records from Celltypist

We still need to add the renaming 4 High hierarchy terms:

list(high_terms_umapped)

['Erythroid', 'B-cell lineage', 'T cells', 'Cycling cells']

Let’s get the top hits from a search:

for term in list(high_terms_umapped):
    print(f"Term: {term}")
    display(celltype_bt.search(term).head(1))

Term: Erythroid

	ontology_id	definition	synonyms	parents	__agg__	__ratio__
name
erythrocyte	CL:0000232	A Red Blood Cell. In Mammals, Mature Erythrocy...	RBC|red blood cell	[CL:0000764]	erythrocyte	70.0

Term: B-cell lineage

	ontology_id	definition	synonyms	parents	__agg__	__ratio__
name
obsolete cell by lineage	CL:0000220	None	None	[]	obsolete cell by lineage	73.684211

Term: T cells

	ontology_id	definition	synonyms	parents	__agg__	__ratio__
name
T cell	CL:0000084	A Type Of Lymphocyte Whose Defining Characteri...	T-lymphocyte|T-cell|T lymphocyte	[CL:0000542]	t cell	92.307692

Term: Cycling cells

	ontology_id	definition	synonyms	parents	__agg__	__ratio__
name
circulating cell	CL:0000080	A Cell Which Moves Among Different Tissues Of ...	None	[]	circulating cell	75.862069

So we decide to:

• Add the “T cells” to the synonyms of the public “T cell” record

• Create the remaining 3 terms only using their names (we think “B cell lineage” shouldn’t be identified with “B cell”)

for name in high_terms_umapped:
    if name == "T cells":
        record = lb.CellType.from_bionty(name="T cell")
        record.add_synonym(name)
        record.save()
    else:
        record = lb.CellType(name=name)
        record.save()
    records_names[name] = record

✅ validated 1 CellType record from Bionty on name: T cell

💡 also saving parents of CellType(id='BxNjby0x', name='T cell', ontology_id='CL:0000084', synonyms='T lymphocyte|T-lymphocyte|T cells|T-cell', description='A Type Of Lymphocyte Whose Defining Characteristic Is The Expression Of A T Cell Receptor Complex.', updated_at=2023-08-12 05:42:22, bionty_source_id='Le97', created_by_id='DzTjkKse')

✅ validated 1 CellType record on ontology_id: CL:0000542

🔶 records with similar names exist! did you mean to load one of them?

	id	synonyms	__ratio__
name
Cycling B cells	ibzfn1zQ		92.857143
Cycling T cells	TTziQpub		92.857143
Cycling NK cells	rC47wc9h		89.655172

Now let’s add the parent records:

for _, row in celltypist_df.iterrows():
    record = records_names[row["name"]]
    if row["parent"] is not None:
        parent_record = records_names[row["parent"]]
        record.parents.add(parent_record)

Access the in-house CellType registry

The previously added CellTypist ontology registry is now available in LaminDB. To retrieve the full ontology table as a Pandas DataFrame we can use .filter:

lb.CellType.filter().df()

	name	ontology_id	abbr	synonyms	description	bionty_source_id	updated_at	created_by_id
id
k9BIIrzE	DC2	CL:0000990	None	None	conventional type 2 dendritic cells which cons...	None	2023-08-12 05:42:13	DzTjkKse
9YpF6UDm	Pre-pro-B cells	CL:0002046	None	None	the earliest primitive B cell progenitors whic...	None	2023-08-12 05:42:13	DzTjkKse
kFIm0X2K	Mono-mac	CL:0000113	None	None	mononuclear phagocytes including monocytes and...	None	2023-08-12 05:42:13	DzTjkKse
xNFx21nO	Double-negative thymocytes	CL:0002489	None	None	the early immature thymocytes from the thymus ...	None	2023-08-12 05:42:13	DzTjkKse
loo3Xanl	common myeloid progenitor	CL:0000049	None	common myeloid precursor|CMP	A Progenitor Cell Committed To Myeloid Lineage...	Le97	2023-08-12 05:42:13	DzTjkKse
...	...	...	...	...	...	...	...	...
9JGbXeUA	dendritic cell	CL:0000451	None	None	A Cell Of Hematopoietic Origin, Typically Resi...	Le97	2023-08-12 05:42:21	DzTjkKse
LO5HDkMx	Erythroid	None	None	None	None	None	2023-08-12 05:42:22	DzTjkKse
mCI0FHW2	B-cell lineage	None	None	None	None	None	2023-08-12 05:42:22	DzTjkKse
BxNjby0x	T cell	CL:0000084	None	T lymphocyte|T-lymphocyte|T cells|T-cell	A Type Of Lymphocyte Whose Defining Characteri...	Le97	2023-08-12 05:42:22	DzTjkKse
9fFQGmnd	Cycling cells	None	None	None	None	None	2023-08-12 05:42:22	DzTjkKse

132 rows × 8 columns

This enables us to look for cell types by creating a lookup object from our new CellType registry.

db_lookup = lb.CellType.lookup()

db_lookup.memory_b_cell

CellType(id='67zMsufW', name='memory B cell', ontology_id='CL:0000787', synonyms='memory B-lymphocyte|memory B lymphocyte|Memory B cells|memory B-cell', description='A Memory B Cell Is A Mature B Cell That Is Long-Lived, Readily Activated Upon Re-Encounter Of Its Antigenic Determinant, And Has Been Selected For Expression Of Higher Affinity Immunoglobulin. This Cell Type Has The Phenotype Cd19-Positive, Cd20-Positive, Mhc Class Ii-Positive, And Cd138-Negative.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')

See cell type hierarchy:

db_lookup.memory_b_cell.view_parents()

Access parents of a record:

db_lookup.memory_b_cell.parents.all()

<QuerySet [CellType(id='0I51jgPp', name='mature B cell', ontology_id='CL:0000785', synonyms='mature B lymphocyte|mature B-cell|mature B-lymphocyte', description='A B Cell That Is Mature, Having Left The Bone Marrow. Initially, These Cells Are Igm-Positive And Igd-Positive, And They Can Be Activated By Antigen.', updated_at=2023-08-12 05:42:16, bionty_source_id='Le97', created_by_id='DzTjkKse'), CellType(id='cx8VcggA', name='B cell', ontology_id='CL:0000236', synonyms='B-cell|B cells|B lymphocyte|B-lymphocyte', description='A Lymphocyte Of B Lineage That Is Capable Of B Cell Mediated Immunity.', updated_at=2023-08-12 05:42:13, bionty_source_id='Le97', created_by_id='DzTjkKse')]>

db_lookup.memory_b_cell.parents.all()[1].parents.all()

<QuerySet [CellType(id='Z0yFV7vU', name='lymphocyte of B lineage', ontology_id='CL:0000945', description='A Lymphocyte Of B Lineage With The Commitment To Express An Immunoglobulin Complex.', updated_at=2023-08-12 05:42:17, bionty_source_id='Le97', created_by_id='DzTjkKse')]>

Annotate a dataset with cell types using CellTypist

Annotate cell types predicted with CellTypist

We now demonstrate how simple it is to predict and add cell types to LaminDB with CellTypist. Our dataset of choice is a simple sample dataset together with a sample model.

input_file = celltypist.samples.get_sample_csv()
input_file

'/opt/hostedtoolcache/Python/3.9.17/x64/lib/python3.9/site-packages/celltypist/data/samples/sample_cell_by_gene.csv'

predictions = celltypist.annotate(
    input_file, model="Immune_All_Low.pkl", majority_voting=True
)

🔎 No available models. Downloading...

📜 Retrieving model list from server https://celltypist.cog.sanger.ac.uk/models/models.json

📚 Total models in list: 19

📂 Storing models in /home/runner/.celltypist/data/models

💾 Downloading model [1/19]: Immune_All_Low.pkl

💾 Downloading model [2/19]: Immune_All_High.pkl

💾 Downloading model [3/19]: Adult_Mouse_Gut.pkl

💾 Downloading model [4/19]: Autopsy_COVID19_Lung.pkl

💾 Downloading model [5/19]: COVID19_HumanChallenge_Blood.pkl

💾 Downloading model [6/19]: COVID19_Immune_Landscape.pkl

💾 Downloading model [7/19]: Cells_Fetal_Lung.pkl

💾 Downloading model [8/19]: Cells_Intestinal_Tract.pkl

💾 Downloading model [9/19]: Cells_Lung_Airway.pkl

💾 Downloading model [10/19]: Developing_Human_Brain.pkl

💾 Downloading model [11/19]: Developing_Human_Thymus.pkl

💾 Downloading model [12/19]: Developing_Mouse_Brain.pkl

💾 Downloading model [13/19]: Healthy_COVID19_PBMC.pkl

💾 Downloading model [14/19]: Human_IPF_Lung.pkl

💾 Downloading model [15/19]: Human_Lung_Atlas.pkl

💾 Downloading model [16/19]: Human_PF_Lung.pkl

💾 Downloading model [17/19]: Lethal_COVID19_Lung.pkl

💾 Downloading model [18/19]: Nuclei_Lung_Airway.pkl

💾 Downloading model [19/19]: Pan_Fetal_Human.pkl

📁 Input file is '/opt/hostedtoolcache/Python/3.9.17/x64/lib/python3.9/site-packages/celltypist/data/samples/sample_cell_by_gene.csv'

⏳ Loading data

🔬 Input data has 559 cells and 32786 genes

🔗 Matching reference genes in the model

🧬 5313 features used for prediction

⚖️ Scaling input data

🖋️ Predicting labels

✅ Prediction done!

👀 Can not detect a neighborhood graph, will construct one before the over-clustering

⛓️ Over-clustering input data with resolution set to 5

🗳️ Majority voting the predictions

✅ Majority voting done!

Now that we’ve predicted all cell types we create an Anndata object that we will eventually track with LaminDB.

adata_annotated = predictions.to_adata()

adata_annotated.obs

	predicted_labels	over_clustering	majority_voting	conf_score
Cell_1	Intermediate macrophages	3	Age-associated B cells	0.979577
Cell_2	Trm cytotoxic T cells	3	Age-associated B cells	0.073008
Cell_3	pDC	9	Macrophages	0.020744
Cell_4	Follicular B cells	36	Age-associated B cells	0.167273
Cell_5	Trm cytotoxic T cells	36	Age-associated B cells	0.430877
...	...	...	...	...
Cell_555	Alveolar macrophages	5	Alveolar macrophages	0.152075
Cell_556	Alveolar macrophages	0	Alveolar macrophages	0.901491
Cell_557	Tcm/Naive helper T cells	5	Alveolar macrophages	0.092006
Cell_558	Alveolar macrophages	5	Alveolar macrophages	0.747148
Cell_559	Alveolar macrophages	0	Alveolar macrophages	0.060108

559 rows × 4 columns

Let’s rename the column with predictions:

adata_annotated.obs.rename(
    columns={"predicted_labels": "cell_type_cell_typist"}, inplace=True
)

Create cell type records:

celltypes = lb.CellType.from_values(
    adata_annotated.obs.cell_type_cell_typist, lb.CellType.name
)

✅ validated 19 CellType records on name: Age-associated B cells, CD16+ NK cells, CD16- NK cells, Cycling T cells, DC, DC2, Double-positive thymocytes, Erythrophagocytic macrophages, Intermediate macrophages, MNP, Mono-mac, NKT cells, T(agonist), Tcm/Naive helper T cells, Tem/Effector helper T cells, Tem/Temra cytotoxic T cells, Trm cytotoxic T cells, Type 17 helper T cells, pDC

🔶 did not validate 11 CellType records for names: Alveolar macrophages, B cells, Classical monocytes, Follicular B cells, Macrophages, Memory B cells, Monocytes, Myelocytes, NK cells, Non-classical monocytes, Regulatory T cells

celltypes[:2]

[CellType(id='00ieV0IG', name='Age-associated B cells', ontology_id='CL:0000787', description='CD11c+ T-bet+ memory B cells associated with autoimmunity and aging', updated_at=2023-08-12 05:42:13, created_by_id='DzTjkKse'),
 CellType(id='4GyMgqBz', name='CD16+ NK cells', ontology_id='CL:0000623', description='CD16+ granular lymphocytes that play protective roles against both infectious pathogens and cancer using antibody-dependent cell-mediated cytotoxicity', updated_at=2023-08-12 05:42:13, created_by_id='DzTjkKse')]

Track the annotated dataset in LaminDB

Create a file record of the AnnData object. We further define a name of the dataset for clarity that can also be queried for.

file_annotated = ln.File.from_anndata(
    adata_annotated, description="Examplary CellTypist file", var_ref=lb.Gene.symbol
)

💡 file will be copied to default storage upon `save()` with key 'Uz07x8DBWfjY7FvsmVjJ.h5ad'

💡 parsing feature names of X stored in slot 'var'

💡    using global setting species = human

✅    validated 24688 Gene records from Bionty on symbol: MIR1302-10, MIR1302-10, FAM138A, OR4F5, OR4F29, OR4F16, FAM87B, LINC00115, FAM41C, SAMD11, NOC2L, KLHL17, PLEKHN1, HES4, ISG15, ISG15, AGRN, AGRN, RNF223, C1orf159, ...

🔶    ambiguous validation in Bionty for 2666 records: MIR1302-10, ISG15, AGRN, TNFRSF4, B3GALT6, PLCH2, PANK4, HES5, TNFRSF14, MMEL1, TTC34, SMIM1, ESPN, PLEKHG5, ENO1, PIK3CD, KIF1B, TARDBP, MASP2, MTOR, ...

🔶    did not validate 13393 Gene records for symbols: AAED1, AARS, AATK-AS1, AB019440.50, AC000003.2, AC000032.2, AC000036.4, AC000067.1, AC000068.10, AC000068.5, AC000068.9, AC000099.1, AC000111.3, AC000111.6, AC000123.4, AC000124.1, AC000370.2, AC002056.5, AC002064.4, AC002064.5, ...

🔶    ignoring non-validated features: 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9B16.1,RP5-899B16.2,RP5-899E9.1,RP5-901A4.1,RP5-902P8.10,RP5-902P8.12,RP5-903G2.2,RP5-905G11.3,RP5-905H7.8,RP5-905H7.9,RP5-905N1.2,RP5-906A24.2,RP5-907D15.3,RP5-908M14.5,RP5-914P20.5,RP5-916L7.2,RP5-919F19.5,RP5-921G16.1,RP5-921G16.2,RP5-928E24.2,RP5-930J4.2,RP5-930J4.4,RP5-933E2.1,RP5-933K21.3,RP5-935K16.1,RP5-936J12.1,RP5-937E21.8,RP5-940F7.2,RP5-940J5.3,RP5-940J5.6,RP5-940J5.8,RP5-940J5.9,RP5-942I16.1,RP5-943J3.2,RP5-944M2.1,RP5-944M2.2,RP5-944M2.3,RP5-945F2.2,RP5-945F2.3,RP5-945I17.2,RP5-947P14.1,RP5-951N9.1,RP5-952N6.1,RP5-955M13.4,RP5-956O18.2,RP5-956O18.3,RP5-963E22.4,RP5-963E22.5,RP5-964N17.1,RP5-965F6.2,RP5-966M1.6,RP5-968D22.1,RP5-968D22.3,RP5-968J1.1,RP5-968P14.2,RP5-971N18.3,RP5-973M2.2,RP5-973N23.4,RP5-974N19.1,RP5-977B1.11,RP5-977B1.7,RP5-978I12.1,RP5-981O7.2,RP5-983L19.2,RP5-984P4.4,RP5-986I17.2,RP5-988G17.1,RP5-991C6.3,RP5-991G20.1,RP5-991G20.2,RP5-991G20.4,RP5-991O23.1,RP5-994D16.3,RP5-994D16.9,RP5-997D16.2,RP5-997D24.3,RP5-998H6.2,RP5-998N21.4,RP5-998N21.7,RP6-102O10.1,RP6-109B7.2,RP6-109B7.3,RP6-109B7.4,RP6-109B7.5,RP6-114E22.1,RP6-159A1.3,RP6-191P20.4,RP6-1O2.1,RP6-201G10.2,RP6-206I17.1,RP6-206I17.2,RP6-206I17.3,RP6-24A23.3,RP6-24A23.6,RP6-27P15.2,RP6-65G23.1,RP6-65G23.3,RP6-74O6.2,RP6-74O6.3,RP6-74O6.6,RP6-91H8.1,RP6-91H8.2,RP6-91H8.3,RP6-91H8.5,RP6-99M1.2,RP6-99M1.3,RPA3-AS1,RPL34-AS1,RPS17L,RPSAP58,RQCD1,RRNAD1,RSBN1L-AS1,RSG1,RTDR1,RTFDC1,SARS,SBP1,SC22CB-1D7.1,SCXA,SCXB,SDCCAG3,SDIM1,SDPR,SELK,SELM,SELO,SELRC1,SELT,SELV,SEP15,SEPN1,SEPP1,SEPT1,SEPT10,SEPT11,SEPT12,SEPT14,SEPT2,SEPT3,SEPT4,SEPT5,SEPT6,SEPT7,SEPT8,SEPT9,SEPW1,SETD5-AS1,SETD8,SF3B14,SGK223,SGK494,SGOL1,SGOL1-AS1,SGOL2,SHFM1,SKIV2L,SKIV2L2,SLC26A10,SLC2A1-AS1,SLC35E2,SLC9A3R1,SLC9A3R2,SLMO1,SLMO2,SMC5-AS1,SMEK1,SMEK2,SMIM4,SNORA76,SNORD45,SOGA2,SPACA6P-AS,SPANXB2,SPATA31A2,SPATA31A4,SPERT,SPG20,SPG20OS,SPHAR,SQRDL,SRCRB4D,SRP14-AS1,SRPR,SSFA2,SSSCA1,SSSCA1-AS1,ST5,STK4-AS1,STRA13,STT3A-AS1,SUV420H1,SUV420H2,T,TAPSAR1,TARS,TARSL2,TAZ,TBC1D29,TBC1D4-AS1,TBCE-AS1,TCEB1,TCEB2,TCEB3,TCEB3B,TCEB3C,TCEB3CL,TCEB3CL2,TCP10,TCTE3,TCTEX1D1,TCTEX1D2,TCTEX1D4,TENC1,TEX40,TGIF2-C20orf24,TIAF1,TLDC1,TM4SF2,TMCC1-AS1,TMEM110,TMEM110-MUSTN1,TMEM133,TMEM136,TMEM14E,TMEM155,TMEM159,TMEM161B-AS1,TMEM173,TMEM180,TMEM189,TMEM189-UBE2V1,TMEM194A,TMEM194B,TMEM2,TMEM206,TMEM211,TMEM246,TMEM251,TMEM257,TMEM261,TMEM27,TMEM30C,TMEM35,TMEM5,TMEM5-AS1,TMEM55A,TMEM55B,TMEM56,TMEM56-RWDD3,TMEM57,TMEM66,TMEM75,TMEM8A,TMEM8C,TMEM99,TMPRSS4-AS1,TMX2-CTNND1,TNKS2-AS1,TNRC6C-AS1,TOLLIP-AS1,TOMM70A,TOPORS-AS1,TPRX2P,TRAPPC2P1,TROVE2,TSRM,TSSC1,TSTA3,TTC18,TTC37,TTC40,TTTY15,TTTY16,TUSC5,TWISTNB,TXNRD3NB,U47924.27,U47924.29,U47924.30,U47924.31,U51244.2,U52111.14,U66059.58,U73166.2,U82670.4,U82695.10,U82695.9,U91319.1,U91324.1,U91328.19,U91328.20,U91328.21,U91328.22,U95743.1,UBA6-AS1,UBE2E2-AS1,UBE2Q2L,UBL7-AS1,UBXN10-AS1,UCHL1-AS1,UFD1L,UHRF1BP1,UHRF1BP1L,UPK3BL,USMG5,USP12-AS2,USP27X-AS1,USP46-AS1,UTP11L,VARS,VIMP,VNN3,VPRBP,VWA9,WAPAL,WARS,WBP5,WBSCR16,WBSCR17,WBSCR22,WBSCR27,WBSCR28,WDR11-AS1,WDR16,WDR34,WDR52,WDR52-AS1,WDR60,WDR61,WDR63,WDR65,WDR66,WDR78,WDR92,WDR96,WDYHV1,WHSC1,WHSC1L1,WI2-1959D15.1,WI2-2373I1.2,WI2-3308P17.2,WI2-80269A6.1,WI2-81516E3.1,WI2-85898F10.1,WI2-87327B8.1,WI2-925H4.1,WIBG,WISP1,WISP2,WISP3,WRB,XAGE1C,XAGE1D,XAGE1E,XAGE2B,XRCC6BP1,XX-C2158C12.1,XX-C2158C12.2,XX-C2158C6.1,XX-C2158C6.3,XX-C283C717.1,XX-CR54.1,XX-CR54.3,XX-DJ76P10__A.2,XX-FW80269A6.1,XX-FW81066F1.2,XX-FW83128A1.2,XXbac-B135H6.15,XXbac-B135H6.18,XXbac-B33L19.3,XXbac-B33L19.4,XXbac-B33L19.6,XXbac-B444P24.14,XXbac-B444P24.8,XXbac-B476C20.17,XXbac-B476C20.9,XXbac-BCX196D17.5,XXbac-BPG13B8.10,XXbac-BPG154L12.4,XXbac-BPG170G13.32,XXbac-BPG181B23.7,XXbac-BPG181M17.5,XXbac-BPG181M17.6,XXbac-BPG248L24.13,XXbac-BPG249D20.9,XXbac-BPG252P9.10,XXbac-BPG252P9.9,XXbac-BPG254F23.7,XXbac-BPG27H4.8,XXbac-BPG299F13.14,XXbac-BPG299F13.17,XXbac-BPG308J9.3,XXbac-BPG308K3.5,XXbac-BPG308K3.6,XXbac-BPG32J3.20,XXbac-BPG55C20.7,XXbac-BPGBPG55C20.1,XXbac-BPGBPG55C20.2,XXbac-BPGBPG55C20.3,XXcos-LUCA11.4,XXcos-LUCA11.5,XXyac-YM21GA2.3,XXyac-YM21GA2.4,XXyac-YM21GA2.7,XXyac-YR29IB3.1,XXyac-YX155B6.5,XXyac-YX155B6.6,XXyac-YX155B6.7,XXyac-YX60D10.1,XXyac-YX65C7_A.2,XXyac-YX65C7_A.3,YAE1D1,YARS,YTHDF3-AS1,Z69666.2,Z69720.2,Z82214.2,Z82214.3,Z83001.1,Z83307.3,Z83844.1,Z83851.1,Z83851.3,Z83851.4,Z97053.1,Z98049.1,Z99756.1,ZADH2,ZBED6CL,ZCCHC11,ZCCHC16,ZCCHC5,ZCCHC6,ZFYVE20,ZHX1-C8ORF76,ZMYM6NB,ZNF205-AS1,ZNF259,ZNF582-AS1,ZNF645,ZNF720,ZNF724P,ZNF788,ZNF812,ZNRD1,ZNRD1-AS1,ZRSR1,ZSCAN5D,ZUFSP,hsa-mir-1199,hsa-mir-150,hsa-mir-335,hsa-mir-490,hsa-mir-7515,hsa-mir-8072,snoU13

🌱    linked: FeatureSet(id='JSKaIUo7fOmFqih2lxZo', n=24688, type='float', registry='bionty.Gene', hash='8qB_C76hV5vB8e5Co8pm', created_by_id='DzTjkKse')

💡 parsing feature names of slot 'obs'

🔶    did not validate 4 Feature records for names: cell_type_cell_typist, over_clustering, majority_voting, conf_score

🔶    ignoring non-validated features: cell_type_cell_typist,over_clustering,majority_voting,conf_score

🔶    no validated features, skip creating feature set

file_annotated.save()

🌱 saved 1 feature set for slot: ['var']

🌱 storing file 'Uz07x8DBWfjY7FvsmVjJ' with key '.lamindb/Uz07x8DBWfjY7FvsmVjJ.h5ad'

ln.save(celltypes)

Add cell types as labels for a feature "cell_type_cell_typist":

ln.Feature(name="cell_type_cell_typist", type="category").save()

file_annotated.add_labels(celltypes)

🌱 linked labels 'Age-associated B cells', 'CD16+ NK cells', 'CD16- NK cells', 'Cycling T cells', 'DC', 'DC2', 'Double-positive thymocytes', 'Erythrophagocytic macrophages', 'Intermediate macrophages', 'MNP', 'Mono-mac', 'NKT cells', 'T(agonist)', 'Tcm/Naive helper T cells', 'Tem/Effector helper T cells', 'Tem/Temra cytotoxic T cells', 'Trm cytotoxic T cells', 'Type 17 helper T cells', 'pDC', 'Alveolar macrophages', 'B cells', 'Classical monocytes', 'Follicular B cells', 'Macrophages', 'Memory B cells', 'Monocytes', 'Myelocytes', 'NK cells', 'Non-classical monocytes', 'Regulatory T cells' to feature 'cell_type_cell_typist', linked feature 'cell_type_cell_typist' to registry 'bionty.CellType'

🌱 created feature set for slot 'external'

file_annotated.describe()

💡 File(id=Uz07x8DBWfjY7FvsmVjJ, key=None, suffix=.h5ad, accessor=AnnData, description=Examplary CellTypist file, version=None, size=75080752, hash=nGDgBNcnPz4y3qSDIiMbEZ, hash_type=sha1-fl, created_at=2023-08-12 05:43:50.501926+00:00, updated_at=2023-08-12 05:43:50.501951+00:00)

Provenance:
    🗃️ storage: Storage(id='ATyyz7R2', root='/home/runner/work/lamin-usecases/lamin-usecases/docs/celltypist', type='local', updated_at=2023-08-12 05:42:07, created_by_id='DzTjkKse')
    📎 initial_version: None
    📔 transform: Transform(id='s5mkN5NQ1ttIz8', name='Manage a cell type registry', short_name='celltypist', stem_id='s5mkN5NQ1ttI', version='0', type='notebook', updated_at=2023-08-12 05:43:47, created_by_id='DzTjkKse')
    🚗 run: Run(id='UKB2bEgIHN2115EtV0it', run_at=2023-08-12 05:42:11, transform_id='s5mkN5NQ1ttIz8', created_by_id='DzTjkKse')
    👤 created_by: User(id='DzTjkKse', handle='testuser1', email='testuser1@lamin.ai', name='Test User1', updated_at=2023-08-12 05:42:07)
Features:
  🗺️ var (X):
    🔗 index (24688, bionty.Gene.id): ['w45ip4X96uaN', '87eyq64qsa5a', 'LeSn2ItoRqLP', '9d4RgqJu60sN', 'rU0IB0y07koI'...]
  🗺️ external:
    🔗 cell_type_cell_typist (30, bionty.CellType): ['DC2', 'Mono-mac', 'T(agonist)', 'Type 17 helper T cells', 'Memory B cells']

file_annotated.view_lineage()

Now we can track the file and search for it for usecase by querying for a specific cell type.

ln.File.filter(cell_types=db_lookup.tcm_naive_helper_t_cells).df()

	storage_id	key	suffix	accessor	description	version	initial_version_id	size	hash	hash_type	transform_id	run_id	updated_at	created_by_id
id
Uz07x8DBWfjY7FvsmVjJ	ATyyz7R2	None	.h5ad	AnnData	Examplary CellTypist file	None	None	75080752	nGDgBNcnPz4y3qSDIiMbEZ	sha1-fl	s5mkN5NQ1ttIz8	UKB2bEgIHN2115EtV0it	2023-08-12 05:43:50	DzTjkKse

Or track in which notebook the file is annotated by celltypist:

ln.Transform.filter(files__description__icontains="CellTypist").df()

	name	short_name	stem_id	version	type	reference	updated_at	created_by_id
id
s5mkN5NQ1ttIz8	Manage a cell type registry	celltypist	s5mkN5NQ1ttI	0	notebook	None	2023-08-12 05:43:47	DzTjkKse

Try it yourself

This notebook is available at laminlabs/lamin-usecases.

Show code cell content Hide code cell content

!lamin delete celltypist
!rm -r ./celltypist

💡 deleting instance testuser1/celltypist
✅     deleted instance settings file: /home/runner/.lamin/instance--testuser1--celltypist.env

✅     instance cache deleted
✅     deleted '.lndb' sqlite file
🔶     consider manually delete your stored data: /home/runner/work/lamin-usecases/lamin-usecases/docs/celltypist